Hands-on Complete HPLC & LC-MS
This course provides hands-on training covering HPLC & LC-MS theory and methods, maintenance and troubleshooting.
|Dates||25 Feb 2019 - 1 Mar 2019 - This event is in the past.|
|Venue||The Open University, Milton Keynes|
|Organiser name||Anthias Consulting Ltd|
This course provides the theory of HPLC and LC-MS essential to any participant, along with the hands-on practical element in the laboratory to practice and re-enforce the theoretical knowledge.
After an introduction to liquid chromatography, this course addresses solvents & buffers (the mobile phase), pumps, isocratic and gradient elution, sample introduction, analytical columns, HPLC detectors, mass spectrometry including tuning, ionisation, mass analysers, ion detector and data analysis. The knowledge is then used to create methods, perform injections and change parameters to see the effects. The course discusses the need for maintenance along with practicals to carry out maintenance on both an HPLC and an LC-MS system such as the autosampler, injector, columns, detectors, MS tuning, vacuum system, ion source and the mass analyser. A day is spent on troubleshooting an HPLC or LC-MS instrument and learning what problems can occur, with real problem solving on the instruments and data analysis software.
Experts in analytical chemistry consulting and training. Our training covers the full expanse of analytical chemistry, including chromatography, mass spectrometry and spectroscopy.