Opportunities for permanent roles in a range of business sectors

We are hiring Research Associates

4basebio is an innovative gene synthesis company with the ambition to provide high quality, synthetic DNA for use in gene therapies and gene vaccines. This is a disruptive technology with tremendous potential. The company is growing rapidly and is establishing a GMP manufacturing and process development hub at our site just north of Cambridge, UK, to facilitate the scaling and optimisation of our proprietary DNA manufacturing process. 

We have several opportunities and are looking to hear from Research Associates with experience with in any of the following skill sets:

DNA purification techniques

  • Precipitation
  • Proficient in use of AKTA FPLC systems
  • IEX, HIC, Affinity chromatography
  • Tangential Flow filtration (TFF)/ cross flow filtration

DNA/RNA synthesis/ analysis

  • Rolling circle DNA amplification
  • qPCR and RT-qPCR
  • DNA Ligation
  • DNA Restriction enzymes
  • Molecular biology and DNA construct design
  • Cloning, transformations and E. coli fermentation
  • Plasmid maxi preps
  • In vitro transcription
  • RNA purification
  • DNA analysis:
    • AGE
    • Qubit
    • UV/VIS
    • Mass Spec
    • HPLC
    • Blotting

Tissue culture/nanoparticles

  • Extensive tissue culture experience in relevant biological models
  • Nanoparticle formulation/microfluidics
  • Nanoparticle characterisation (DLS, microscopy, nano-tracking analysis)
  • ELISAs
  • Western blotting
  • In vivo experience

Gene editing

  • Experience in gene editing techniques such as CRISPR/Cas9, TALENs
  • Construct design of repair templates
  • Application of editing strategies in mammalian cells/ primary cells
  • Delivery mechanisms- viral non-viral or electroporation

AAV manufacture

  • AAV production
  • AAV purification
  • AAV characterisation – TCID50, full:empty ratio, ELISA, RTqPCR

You must hold a Degree in Life Sciences or Biotechnology. 

We can only consider candidates who hold the right to work in the UK/indefinite leave to stay.

In return we offer a competitive salary and a great benefits package.

If you would like to know more, int he first instance, send you CV to HR@4basebio.com