Crystallising the truth


TTP LabTech today announced the availability for download of an application note on mosquito® Crystal: key for identifying the structure of intracellular glutathione transport protein.

The note is entitled “mosquito® Crystal for the rapid screening and optimisation of crystallisation conditions of the bacterial periplasmic binding protein, HbpA and the elucidation of its role as a glutathione import protein”. The revolutionary design of the mosquito® Crystal offers accurate and rapid nanolitre dispensing of even the most viscous liquids (from 50 nL to 1.2 µL). In addition, its low cost disposable tips guarantee zero cross contamination between samples, making mosquito Crystal the ideal platform for flexible dispensing in crystallography.

Glutathione is one of the most important antioxidant molecules produced by the body. With roles extending from growth and repair to molecular detoxification and excretion, it is ubiquitous in all cells whether eukaryotic or prokaryotic. Although GSH is made constitutively within eukaryotic cells, bacteria are auxotrophic for GSH and need to acquire this molecule by import mechanisms.

Bacteria which are unable to import GSH are more susceptible to oxidative and disulphide stress, reducing their pathogenicity and ability to colonise certain host niches within the body. An understanding of such bacterial import proteins may therefore be useful for understanding mechanisms of pathogenicity and infection. This application note describes how the mosquito Crystal was used to dispense 100 nL crystallisation droplets (50 nL protein sample and 50 nL crystallisation condition). This allowed rapid and efficient screening of almost 2000 crystallisation conditions, ultimately resulting in the successful crystallisation and identification of a novel GSH import protein.

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To learn more about the mosquito® Crystal nanolitre pipettor, please visit:

About TTP LabTech Ltd
Based near Cambridge, UK, TTP LabTech supplies laboratory instrumentation for the healthcare, biotech and pharmaceutical sectors. TTP LabTech’s products are designed to increase efficiency, effectiveness, flexibility or overall capability in key biopharmaceutical research processes including compound storage (comPOUND®), low volume liquid handling (mosquito®), high content screening (Acumen®), antibody discovery (Mirrorball®) and sample analysis management (LAB2LAB). 

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TTP is an independent technology company where scientists and engineers collaborate to invent, design and develop new products and technologies. Working across a wide spectrum of industries including health, telecoms, industrials and consumer, TTP creates breakthrough solutions that bring strong commercial value to clients and the benefits of technology to all.

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