A new multinational study has shown how the process of distinguishing viruses and bacteria could be accelerated through the use of computational methods.
Identification of viruses and bacteria could be sped up through computational methods
The researchers, led by the University of Edinburgh, with colleagues from Cambridge, London, Slovenia and China, used a combination of theoretical and experimental methods to develop a strategy to detect the DNA of infectious diseases. The results are reported in the Proceedings of the National Academy of Sciences.
The current coronavirus pandemic highlights the need for fast and accurate detection of infectious diseases. Importantly, viral infections like coronavirus and bacterial infections like those associated with antimicrobial resistance (AMR) need to be distinguished. This is usually done by using a complementary sequence that binds selectively to the genome of interest. Normally, this is done by targeting a single, long DNA sequence that is unique to the pathogen.
However, the researchers believe that much higher selectivities can be achieved by simultaneously targeting many shorter sequences that occur with a higher frequency in the pathogen of interest than in the DNA of other organisms that may be present in the patient samples.
"This approach exploits a phenomenon called ‘multivalency’, and the extensive numerical calculations, based on real bacterial and viral DNA sequences show that this approach should significantly outperform current approaches," said co-author Professor Erika Eiser from Cambridge’s Cavendish Laboratory. "Even though the individual shorter sequences bind more weakly to the target DNA than a single, longer sequences, the strength of the multivalent binding increases much faster than linearly with the number of short sequences."
In other words, instead of designing molecular probes that bind strongly to one place on the target DNA, researchers should, counterintuitively, design probes that bind weakly all over the target DNA. Making such relatively short probe sequences is, at present, a standard procedure and the sequences can be ordered online.
Reproduced courtesy of the University of Cambridge
The University of Cambridge is acknowledged as one of the world's leading higher education and research institutions. The University was instrumental in the formation of the Cambridge Network and its Vice- Chancellor, Professor Stephen Toope, is also the President of the Cambridge Network.