Targets such as GPCRs, ion channels, multi-pass transmembrane proteins, and multi-component systems like CD3–TCR frequently fail early in discovery workflows. This is often driven by the fact that target presentation and biological environment determine which conformations and epitopes are accessible. The result is a familiar failure mode: selections enrich the wrong binders, and functional screens produce misleading readouts that do not translate downstream.
This session introduces a practical framework to address these issues earlier in discovery. By aligning target selection, biological context, and mechanism of action from the outset, teams can reduce false positives, improve interpretation of screening data, and make more informed progression decisions. Attendees will leave with practical decision criteria for choosing presentation formats and early screening approaches for challenging membrane targets.
Session Takeaways
- De-risk translation between panning and screening: how conformation, environment, and presentation create format-dependent epitope access
- Building your own Nanodisc workflows: practical considerations and example workflows (including CD3–TCR) to preserve native-like structures and improve epitope accessibility for discovery and functional screening
Watch the full webinar on demand: https://youtu.be/unCNI0LMj7w?si=OETSsCEZqznTxYBV
